大熊猫全基因组dna提取方法

Method for extracting hologenome of giant panda

Abstract

The present invention provides a method for extracting holo-genome DNA of giant panda. Said invented method includes the following steps: using ethyl alcohol with different concentrations to make step-by-step dehydration of material for extracting DNA, precipitation separation, critical drying, removing ethyl alcohol or formalin, pulverizing, adding protease and SDS and digesting, using phenol and chloroform to make extraction, using cold ethyl alcohol to make precipitation and vacuum drying. Said invention solves the material source for extracting DNA of giant panda and other wild and pen-grown animals, and can be used for population genetic research of animal population and gene analysis of fixed test material of medical difficult and complicated cases.
本发明提供一种大熊猫全基因组DNA提取方法,包括将DNA提取材料用不同浓度乙醇逐级脱水,沉淀分离,临界干燥,除去乙醇或福尔马林,粉碎,加入蛋白酶和SDS消化,再用苯酚、氯仿提抽,冷乙醇沉淀,真空干燥。该方法解决了大熊猫及其它野生和圈养动物DNA的材料来源,可用于动物种群的群体遗传研究,以及医学上疑难病症的检材固定后作基因分析。

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    CN-102485979-AJune 06, 2012深圳华大基因研究院, 深圳华大基因科技有限公司Ffpe样品核酸文库,其构建方法和ffpe样品分析方法
    WO-2012071985-A1June 07, 2012深圳华大基因科技有限公司, 深圳华大基因研究院Method for extracting dna from ffpe samples and use thereof